PNA probe types
-N-terminus free amine
-N-terminus and C-terminus labeling
Amino acid modified other appropriate functional group modification
Alpha or Gamma PNA
-Multi-labeled PNA backbone
-PNA backbone additional functions
1. PNA has high binding affinity, and 13~18 bases would be adequate.
2. Purine-rich sequences tend to aggregate and have low solubility
-Purine content limit: 80%(It is recommended to keep it lower than 60%)
-Avoid purine stretch over 5 residues
3. To improve solubility, solubility enhancers such as O linker(eg linker), E linker, X linker, lysines, etc. can be incorporated into the base sequence.
4. For N-terminus labeling, add O linker, etc. to PNA and fluorescence labeling to avoid influence of labeled molecules during hybridization.
5. For C-terminus labeling, add lysines or L-cysteines to C-terminus.
Types of labeling materials
Please contact us for more fluorescent labeling materials.